{
    "tag": 8220,
    "title": "Experimental PCR Data on Soil DNA Extracts",
    "pubdate": "20160602",
    "sername": null,
    "series_name": null,
    "issue": "doi:10.5066\/F7WW7FRJ",
    "publish": null,
    "publisher_name": null,
    "onlink": "https:\/\/cmgds.marine.usgs.gov\/catalog\/spcmsc\/banthracis_detection_survey_metadata.faq.html",
    "format": null,
    "email": null,
    "descript": "Bacillus species and B. anthracis presence\/absence data were determined in 4,770 soil samples collected across the contiguous United States, in cooperation with the U.S. Environmental Protection Agency (EPA). Polymerase Chain Reaction (PCR) data for Bacillus species and B. anthracis rpoB gene PCR amplicon detection were reported as non-detect (n), low (l), medium (m), and high (h). Results for both pag and lef genes of the pX01 plasmid were reported by the University of South Florida's Center for Biological Defense. This data was recorded as negative or positive for each of the genes and included the following combinations: neg\/neg, pos\/neg, neg\/pos, and pos\/pos.  Data for the pX02 plasmid were recorded as negative (neg) or positive (pos).",
    "lang": null,
    "journal": null,
    "pwid": null,
    "originator": [
        {
            "name": "Griffin, Dale W.",
            "role": "Author"
        },
        {
            "name": "Douglas, Steven H.",
            "role": "Author"
        }
    ],
    "index_term": [
        {
            "thcode": 2,
            "code": "345",
            "name": "environmental health (human)",
            "scope": "Effects of the environment on human health."
        },
        {
            "thcode": 2,
            "code": "437",
            "name": "geochemistry",
            "scope": "Study of the distribution of chemical elements and natural compounds on the earth and in the atmosphere and the chemical processes that affect the earth."
        },
        {
            "thcode": 2,
            "code": "1081",
            "name": "soil sciences",
            "scope": "Earth sciences dealing with the origin, classification, physical, chemical, and biological properties of soils."
        },
        {
            "thcode": 15,
            "code": "002",
            "name": "biota",
            "scope": "Flora or fauna in natural environment, for example wildlife, vegetation, biological sciences, ecology, wilderness, sea life, wetlands, habitat, biological resources"
        },
        {
            "thcode": 15,
            "code": "007",
            "name": "environment",
            "scope": "Environmental resources, protection and conservation, for example environmental pollution, waste storage and treatment, environmental impact assessment, monitoring environmental risk, nature reserves, landscape, water quality, air quality, environmental modeling"
        },
        {
            "thcode": 15,
            "code": "008",
            "name": "geoscientificInformation",
            "scope": "Information pertaining to earth sciences, for example geophysical features and processes, geology, minerals, sciences dealing with the composition, structure and origin of the earth's rocks, risks of earthquakes, volcanic activity, landslides, gravity information, soils, permafrost, hydrogeology, groundwater, erosion"
        },
        {
            "thcode": 15,
            "code": "009",
            "name": "health",
            "scope": "Health, health services, human ecology, and safety, for example disease and illness, factors affecting health, hygiene, substance abuse, mental and physical health, health services, health care providers, public health"
        }
    ],
    "place_term": [],
    "image": [],
    "fan": []
}